Kmo^tm1.2Pjmi
Targeted Allele Detail

Summary

• Symbol: Kmo^tm1.2Pjmi
• Name: kynurenine 3-monooxygenase; targeted mutation 1.2, Paul J Muchowski
• MGI ID: MGI:5569396
• Gene: Kmo Location: Chr1:175459759-175488419 bp, + strand Genetic Position: Chr1, 81.6 cM
• Alliance: Kmo^tm1.2Pjmi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207199
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the selection cassette and left exon 5 floxed. Cre-mediated recombination removed exon 5. Western blot analysis confirmed the absence of protein expression in the brain and liver. (J:207199)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmo Mutation: 36 strains or lines available

References

• Original: J:207199 Giorgini F, et al., Targeted deletion of kynurenine 3-monooxygenase in mice: a new tool for studying kynurenine pathway metabolism in periphery and brain. J Biol Chem. 2013 Dec 20;288(51):36554-66
• All: 4 reference(s)