Tg(Aire-cre/Esr1)1Mmat
Transgene Detail

Summary

• Symbol: Tg(Aire-cre/Esr1)1Mmat
• Name: transgene insertion 1, Mitsuru Matsumoto
• MGI ID: MGI:5570119
• Synonyms: Aire/CreER BAC tg, Tg(Aire-cre/ERT2)1Mmat
• Transgene: Tg(Aire-cre/Esr1)1Mmat Location: unknown
• Alliance: Tg(Aire-cre/Esr1)1Mmat page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Aire-cre/Esr1)1Mmat expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Aire (MGI:1338803)

• Mutation details: A targeting vector was constructed from a BAC (RP23-461E7) containing the entire Aire gene by replacing the Aire start codon with the open reading frame of a cre/Esr1 (originally reported as ERT2) fusion sequence and the polyA signal of rabbit beta-globin. The linearized BAC construct was microinjected into C57BL/6 fertilized pronuclei and founders were identified by Southern blot analysis. One line was established and crossed to reporter mouse lines for further analyses. (J:209922)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Aire (mouse) Summary of all recombinase alleles driven by Aire. Alliance mouse Aire gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:209922 Nishikawa Y, et al., Temporal lineage tracing of Aire-expressing cells reveals a requirement for Aire in their maturation program. J Immunol. 2014 Mar 15;192(6):2585-92
• All: 1 reference(s)