Dpp9^tm1.1Mdg
Targeted Allele Detail

Summary

• Symbol: Dpp9^tm1.1Mdg
• Name: dipeptidylpeptidase 9; targeted mutation 1.1, Mark D Gorrell
• MGI ID: MGI:5574450
• Synonyms: DPP9^S729A
• Gene: Dpp9 Location: Chr17:56493807-56525905 bp, - strand Genetic Position: Chr17, 29.2 cM
• Alliance: Dpp9^tm1.1Mdg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209219
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Dominant negative)
• Mutations: Nucleotide substitutions, Single point mutation
• Mutation details: A loxP-flanked exon 20 with amino acid substitution of serine to alanine at position 729 (S729A), followed by a FRT-PGK-neo-FRT cassette was inserted by homologous recombination. Flp-mediated recombination removed the neo-cassette. This point mutation results in an enzyme inactive form of the protein. No change in subcellular localization was detected by immunohistochemical analysis. (J:209219)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dpp9 Mutation: 64 strains or lines available

References

• Original: J:209219 Gall MG, et al., Targeted inactivation of dipeptidyl peptidase 9 enzymatic activity causes mouse neonate lethality. PLoS One. 2013;8(11):e78378
• All: 4 reference(s)