Tg(Cck-cre,-lacZ)1Mini
Transgene Detail

Summary

• Symbol: Tg(Cck-cre,-lacZ)1Mini
• Name: transgene insertion 1, Liliana Minichiello
• MGI ID: MGI:5574585
• Synonyms: BAC-Cck-Cre, Cck-cre
• Transgene: Tg(Cck-cre,-lacZ)1Mini Location: unknown
• Alliance: Tg(Cck-cre,-lacZ)1Mini page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)

Transgene description

• Transgene Type: Transgenic (Recombinase, Reporter)
• Mutation: Insertion
• Tg(Cck-cre,-lacZ)1Mini expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cck (MGI:88297)

• Mutation details: A BAC (RP23-26B6) containing the Cck gene was modified by ET recombination so that a cassette composed of a hybrid intron, a cre recombinase gene, an internal ribosomal entry site (IRES), a beta-galactosidase gene (lacZ), and a neomycin/kanamycin resistance marker was inserted into the first ATG of the first coding exon (exon 2). The neo/kan marker was removed using flp recombinase. The purified construct was injected into the pronuclei of (CBA x C57BL/6) hybrid oocytes. (J:210229)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cck (mouse) Summary of all recombinase alleles driven by Cck. Comparative matrix view of recombinase activities Alliance mouse Cck gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:210229 Geibel M, et al., Ablation of TrkB signalling in CCK neurons results in hypercortisolism and obesity. Nat Commun. 2014;5:3427
• All: 2 reference(s)