Esr1^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Esr1^tm1.1Ics
• Name: estrogen receptor 1 (alpha); targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:5574591
• Synonyms: C451A-ERalpha knock-in, ERalpha-C451A
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^tm1.1Ics page

Ovary abnormalities in Esr1^tm1.1Ics/Esr1^tm1.1Ics females

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206373
• Parent Cell Line: BD10 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

Allele Type: Targeted (Not Specified) Mutations: Insertion, Single point mutation Mutation details: A point mutation was introduced, resulted in C451A substitution in the palmitoylation site in exon 7. This mutation is predicted to abrogate membrane localization of the protein. A loxP-flanked neo cassette inserted in intron 7 was removed via Cre-mediated recombination. While protein levels in the uterus, aorta and hepatocytes are similar to wild-type, sucrose gradient isolation of hepatocyte plasma membranes detected a 60% decrease in protein abundance in homozygous mice. (J:206373)

Generation of the Esr1^tm1.1Ics allele

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 68 strains or lines available

References

• Original: J:206373 Adlanmerini M, et al., Mutation of the palmitoylation site of estrogen receptor alpha in vivo reveals tissue-specific roles for membrane versus nuclear actions. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):E283-90
• All: 9 reference(s)