Tbx21^{tm1.1(flpe/ERT2)Gmld}
Targeted Allele Detail

Summary

• Symbol: Tbx21^{tm1.1(flpe/ERT2)Gmld}
• Name: T-box 21; targeted mutation 1.1, Graham M Lord
• MGI ID: MGI:5575639
• Synonyms: T-bet^fl
• Gene: Tbx21 Location: Chr11:96988897-97006157 bp, - strand Genetic Position: Chr11, 60.95 cM, cytoband D
• Alliance: Tbx21^{tm1.1(flpe/ERT2)Gmld} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207104
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tbx21^{tm1.1(flpe/ERT2)Gmld} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbx21 (MGI:1888984)

• Mutation details: A loxP site was inserted upstream of exon 4. An IRES, flpeERT2, polyadenylation sequence, FRT-flanked neomycin resistance cassette and a loxP site were inserted downstream of exon 6. Flp-mediated recombination removed the selection cassette and left exons 4 through 6 floxed. (J:207104)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbx21 (mouse) Summary of all recombinase alleles driven by Tbx21. Alliance mouse Tbx21 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx21 Mutation: 39 strains or lines available

References

• Original: J:207104 Gokmen MR, et al., Genome-wide regulatory analysis reveals that T-bet controls Th17 lineage differentiation through direct suppression of IRF4. J Immunol. 2013 Dec 15;191(12):5925-32
• All: 1 reference(s)