Chd5^tm1.2Gmbr
Targeted Allele Detail

Summary

• Symbol: Chd5^tm1.2Gmbr
• Name: chromodomain helicase DNA binding protein 5; targeted mutation 1.2, Garrett M Brodeur
• MGI ID: MGI:5575642
• Gene: Chd5 Location: Chr4:152423108-152474651 bp, + strand Genetic Position: Chr4, 83.04 cM, cytoband E2
• Alliance: Chd5^tm1.2Gmbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207846
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 12. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 13. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 12 and 13. Western blot analysis confirmed the absence of protein expression in the testes. (J:207846)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chd5 Mutation: 73 strains or lines available

References

• Original: J:207846 Zhuang T, et al., CHD5 is required for spermiogenesis and chromatin condensation. Mech Dev. 2014 Feb;131:35-46
• All: 1 reference(s)