Cxcl12^tm1.1Fass
Targeted Allele Detail

Summary

• Symbol: Cxcl12^tm1.1Fass
• Name: C-X-C motif chemokine ligand 12; targeted mutation 1.1, Fernando Arenzana-Seisdedos
• MGI ID: MGI:5575859
• Synonyms: Cxcl12^deltaex2, Cxcl12^Gagtm1
• Gene: Cxcl12 Location: Chr6:117145496-117158328 bp, + strand Genetic Position: Chr6, 54.81 cM, cytoband F1
• Alliance: Cxcl12^tm1.1Fass page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209558
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with one in which nucleotide substitutions (AAG to TCG and AAA to TCT) result in the amino acid substitution of serine for lysine at positions 24 and 25 (K24S, K25S). A premature stop codon was introduced into exon 4 preventing the translation of the last 30 amino acids. The predicted product is expected to not be able to interact with heparan sulfate structures while not affecting CXCR4-depedent cell signaling. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of exon 3. (J:209558)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cxcl12 Mutation: 25 strains or lines available

References

• Original: J:209558 Rueda P, et al., Homeostatic and tissue reparation defaults in mice carrying selective genetic invalidation of CXCL12/proteoglycan interactions. Circulation. 2012 Oct 9;126(15):1882-95
• All: 3 reference(s)