Insm1^{tm1.1(GFP/cre)Mgn}
Targeted Allele Detail

Summary

• Symbol: Insm1^{tm1.1(GFP/cre)Mgn}
• Name: insulinoma-associated 1; targeted mutation 1.1, Mark A Magnuson
• MGI ID: MGI:5576020
• Synonyms: Insm1(GFP-Cre), Insm1^GFPCre, Insm1^tm1.1Mgn
• Gene: Insm1 Location: Chr2:146064021-146066940 bp, + strand Genetic Position: Chr2, 71.33 cM
• Alliance: Insm1^{tm1.1(GFP/cre)Mgn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Insm1^{tm1.1(GFP/cre)Mgn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Insm1 (MGI:1859980)

• Mutation details: Using recombinase mediated cassette exchange (RMCE) a 532 bp fragment containing a GFP-Cre fusion protein replaced the entire coding region of the gene. The donor construct includes a frt-flanked pgk-hygromycin immediately downstream of the GFP-Cre fusion sequence. The frt-flanked pgk-hygromycin was removed by FLPe mediated recombination. Western blot analysis confirmed absence of protein in homozygotes. Green fluorescence is seen in pancreatic pre-endocrine cells and in islet cells after birth, in the developing central nervous system between E9.5 and E18.5, and also in the peripheral nervous system and gut endocrine cells. (J:94077, J:214095)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Insm1 (mouse) Summary of all recombinase alleles driven by Insm1. Alliance mouse Insm1 gene page

Expression

In Mice Carrying this Mutation:	15 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Insm1 Mutation: 13 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 8 reference(s)