Slc1a2^tm1.1Ncd
Targeted Allele Detail

Summary

• Symbol: Slc1a2^tm1.1Ncd
• Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2; targeted mutation 1.1, NC Danbolt
• MGI ID: MGI:5576462
• Synonyms: EAAT2-flox
• Gene: Slc1a2 Location: Chr2:102489004-102621129 bp, + strand Genetic Position: Chr2, 54.13 cM
• Alliance: Slc1a2^tm1.1Ncd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207177
• Parent Cell Line: D1 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 2 and an frt-neo-frt-loxP cassette was inserted into intron 4, floxing exons 3 and 4 of the Slc1a2 gene from the bacterial artificial chromosome clone RP24-315H4. The neomycin cassette was removed via FLP-mediated recombination, leaving exons 3 and 4 floxed by loxP sites. Cre-mediated recombination can be used to remove exons 3 and 4 which results in a frameshift deletion. (J:207177)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc1a2 Mutation: 40 strains or lines available

References

• Original: J:207177 Zhou Y, et al., Proteome analysis and conditional deletion of the EAAT2 glutamate transporter provide evidence against a role of EAAT2 in pancreatic insulin secretion in mice. J Biol Chem. 2014 Jan 17;289(3):1329-44
• All: 5 reference(s)