Trp63^tm2.2Elrf
Targeted Allele Detail

Summary

• Symbol: Trp63^tm2.2Elrf
• Name: transformation related protein 63; targeted mutation 2.2, Elsa R Flores
• MGI ID: MGI:5576519
• Synonyms: deltaNp63^delta
• Gene: Trp63 Location: Chr16:25502513-25710842 bp, + strand Genetic Position: Chr16, 17.37 cM
• Alliance: Trp63^tm2.2Elrf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206433
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3' and an FRT site flanked neomycin resistance gene cassette and loxP site were inserted downstream of exon 3'. The neo cassette was removed through subsequent flp-mediated recombination. The floxed exon 3' was deleted through cre-mediated recombination. This deletion specifically knocks out the specific deltaN isoform that uses an alternative exon 3 that lacks the acidic transactivation domain sequence. (J:206433)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp63 Mutation: 60 strains or lines available

References

• Original: J:206433 Chakravarti D, et al., Induced multipotency in adult keratinocytes through down-regulation of DeltaNp63 or DGCR8. Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):E572-81
• All: 4 reference(s)