Rab8b^tm1.2Aha
Targeted Allele Detail

Summary

• Symbol: Rab8b^tm1.2Aha
• Name: RAB8B, member RAS oncogene family; targeted mutation 1.2, Akihiro Harada
• MGI ID: MGI:5578141
• Gene: Rab8b Location: Chr9:66750946-66826969 bp, - strand Genetic Position: Chr9, 36.17 cM
• Alliance: Rab8b^tm1.2Aha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211898
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct inserted upstream of exon 2 contain an FRT site, splice acceptor, IRES, beta-geo cassette, polyadenylation sequence, FRT site and loxP. An additional loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the FRT-flanked beta-geo cassette. Cre-mediated recombination removed exon 2. RT-PCR confirmed the absence of transcript expression in the small intestine. (J:211898)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab8b Mutation: 16 strains or lines available

References

• Original: J:211898 Sato T, et al., Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis. J Cell Sci. 2014 Jan 15;127(Pt 2):422-31
• All: 2 reference(s)