Mgat4b^tm1.1Jxm
Targeted Allele Detail

Summary

• Symbol: Mgat4b^tm1.1Jxm
• Name: mannoside acetylglucosaminyltransferase 4, isoenzyme B; targeted mutation 1.1, Jamey Marth
• MGI ID: MGI:5578604
• Synonyms: GnT-IVb deficient, Mgat4b delta, Mgat4b type 1
• Gene: Mgat4b Location: Chr11:50116162-50125930 bp, + strand Genetic Position: Chr11, 30.37 cM
• Alliance: Mgat4b^tm1.1Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212313
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. Cre-mediated recombination removed the neo cassette and exons 2-4 leaving a residual loxP site. Removal of exons 2-4 causes a frameshift at the junction of exon 1 and 5, generating a new translational termination codon that results in a truncated protein lacking the catalytic domain. (J:212313)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mgat4b Mutation: 32 strains or lines available

References

• Original: J:212313 Takamatsu S, et al., Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice. Glycobiology. 2010 Jan;20(4):485-97
• All: 1 reference(s)