Polr2a^{Tn(pb-CAG-GCaMP5g)Tvrd}
Transposon induced Allele Detail

Summary

• Symbol: Polr2a^{Tn(pb-CAG-GCaMP5g)Tvrd}
• Name: polymerase (RNA) II (DNA directed) polypeptide A; transposon insertion 1.1, Petr Tvrdik
• MGI ID: MGI:5578722
• Synonyms: PC::G5
• Gene: Polr2a Location: Chr11:69624823-69649459 bp, - strand Genetic Position: Chr11, 42.86 cM, cytoband B1-C
• Alliance: Polr2a^{Tn(pb-CAG-GCaMP5g)Tvrd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212315
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Transposon induced (Conditional ready, No functional change, Reporter)
• Mutation: Transposon insertion
• Mutation details: The transposon contains a floxed STOP cassette followed by a NEO selection cassette in opposite orientation, the genetically encoded calcium indicator GCaMP5g, an FRT-flanked IRES-tdTomato, and woodchuck hepatitis posttranscriptional regulatory element (WPRE), under the control of the CAG promoter. This construct was flanked by piggyBAC transposase inverted terminal repeats and the entire reporter cassette was inserted approximately 1 kb 3' of the last exon of the Polr2a gene. Flpe mediated recombination removed the FRT-flanked IRES-tdTomato leaving a floxed GCaMP5g. (J:212315)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polr2a Mutation: 92 strains or lines available

References

• Original: J:212315 Gee JM, et al., Imaging activity in neurons and glia with a Polr2a-based and cre-dependent GCaMP5G-IRES-tdTomato reporter mouse. Neuron. 2014 Sep 3;83(5):1058-72
• All: 1 reference(s)