Mcrs1^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Mcrs1^{tm1.1(KOMP)Vlcg}
• Name: microspherule protein 1; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5581465
• Synonyms: Mcrs1^-
• Gene: Mcrs1 Location: Chr15:99140698-99149838 bp, - strand Genetic Position: Chr15, 56.09 cM
• Alliance: Mcrs1^{tm1.1(KOMP)Vlcg} page
• IMPC: Mcrs1 gene page

Mcrs1^{tm1.1(KOMP)Vlcg}/Mcrs1^{tm1.1(KOMP)Vlcg} mice exhibit embryonic lethality, with embryos recovered at E3.5 as blastocysts but no embryos at E7.5. Blastocysts grown in vitro hatch from the zona pellucida but the inner cell mass/epiblast cells fail to form a typical outgrowth colony.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: 16025A-A2
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 7372bp between positions 99073529-99080900 of Chromosome 15 (Genome Build37) and subsequent Cre-mediated excision deleted the neomycin selection cassette. (J:136110, J:188991)

Expression

In Mice Carrying this Mutation:	77 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mcrs1 Mutation: 43 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 7 reference(s)