Cables1^{Tg(CAG-rtTA,CAG-tTS)138Utr}
Transgenic Allele Detail

Summary

• Symbol: Cables1^{Tg(CAG-rtTA,CAG-tTS)138Utr}
• Name: CDK5 and Abl enzyme substrate 1; transgene insertion 138, University of Tsukuba Laboratory Animal Resource Center
• MGI ID: MGI:5581467
• Synonyms: Cables1^TAS, Cables1^{Tg(Actb-rtTA,Actb-tTS)138Utr}, TAS
• Gene: Cables1 Location: Chr18:11972600-12078681 bp, + strand Genetic Position: Chr18, 5.88 cM, cytoband A2
• Alliance: Cables1^{Tg(CAG-rtTA,CAG-tTS)138Utr} page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: RT-PCR analyses, long-range PCR and sequencing analyses showed that the genomic region from chr18:12,058,849 to 12,070,825 (build 38), including exon 4 of Cables1, was deleted at the transgene insertion site. A mutant small protein is able to be translated from the mutant allele in HEK293T cells when transfected with a Cables1-TAS expression vector. However, western blotting analysis with anti-Cables1 antibody (M-280) did not specifically detect wild-type or truncated Cables1 protein in brain lysate. (J:206297)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cables1 Mutation: 40 strains or lines available

References

• Original: J:167073 Mizuno S, et al., A novel locus on proximal chromosome 18 associated with agenesis of the corpus callosum in mice. Mamm Genome. 2010 Dec;21(11-12):525-33
• All: 2 reference(s)