Dnase1l3^tm1Dkit
Targeted Allele Detail

Summary

• Symbol: Dnase1l3^tm1Dkit
• Name: deoxyribonuclease 1-like 3; targeted mutation 1, Daisuke Kitamura
• MGI ID: MGI:5581508
• Synonyms: DNase gamma ^-
• Gene: Dnase1l3 Location: Chr14:14475014-14504586 bp, + strand Genetic Position: Chr14, 4.67 cM, cytoband A1
• Alliance: Dnase1l3^tm1Dkit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211019
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced codon His160 in exon 5, which comprises the catalytic center of the endonuclease. An activity gel assay confirmed the absence of protein activity. (J:211019)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Dnase1l3 Mutation: 24 strains or lines available

References

• Original: J:211019 Mizuta R, et al., DNase gamma is the effector endonuclease for internucleosomal DNA fragmentation in necrosis. PLoS One. 2013;8(12):e80223
• All: 5 reference(s)