Prg4^tm2Mawa
Targeted Allele Detail

Summary

• Symbol: Prg4^tm2Mawa
• Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein); targeted mutation 2, Matthew Warman
• MGI ID: MGI:5581510
• Synonyms: Prg4:GT, Prg4^GT
• Gene: Prg4 Location: Chr1:150325163-150341916 bp, - strand Genetic Position: Chr1, 63.84 cM, cytoband G
• Alliance: Prg4^tm2Mawa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Null/knockout, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: A targeting vector containing a loxP-flanked artifical exon that contains a strong splice acceptor, an in-frame Bgeo lacZ coding sequence, an internal ribosome entry site (IRES), a reverse tet transactivator (rtTA) cassette with a polyA signal, and a neomycin selection cassette driven by its own promoter was inserted into intron 2 of the gene. The allele does not express wild-type Prg4 mRNA, as determined by RT-PCR. Cre-mediated excision of the targeting vector leaves one loxP in intron 2 and allows expression of Prg4 mRNA. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prg4 Mutation: 71 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 5 reference(s)