Myf5^tm2Pas
Targeted Allele Detail

Summary

• Symbol: Myf5^tm2Pas
• Name: myogenic factor 5; targeted mutation 2, Institut Pasteur
• MGI ID: MGI:5581636
• Synonyms: myf-5^a1, Myf5-nlacZ, Myf5/nlacZ, Myf5^nlacZ
• Gene: Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
• Alliance: Myf5^tm2Pas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:16491
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An nLacZ gene is fused in-frame to the DNA sequences encoding the first 13 amino acids introducing a small deletion and resulting in a disruption of the gene and expression of nLacZ under the transcriptional regulation of the endogenous promoter. A TK-neomycin resistance cassette was inserted downstream of the reporter gene. In-situ hybridization analysis indicates that Myf5 and Myf6 expression is absent. (J:16491, J:33706)

Expression

In Mice Carrying this Mutation:	110 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myf5 Mutation: 17 strains or lines available

References

• Original: J:16491 Tajbakhsh S, et al., Mouse limb muscle is determined in the absence of the earliest myogenic factor myf-5. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):747-51
• All: 32 reference(s)