Rcor1^tm2.2Gman
Targeted Allele Detail

Summary

• Symbol: Rcor1^tm2.2Gman
• Name: REST corepressor 1; targeted mutation 2.2, Gail Mandel
• MGI ID: MGI:5581675
• Gene: Rcor1 Location: Chr12:111005801-111082336 bp, + strand Genetic Position: Chr12, 60.93 cM
• Alliance: Rcor1^tm2.2Gman page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211681
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was introduced to intron 3 and an FRT-PGK-Neo-pA-splice donor-FRT-loxP cassette was placed in intron 4. Flpe-mediated recombination removed the FRT-flanked neo cassette leaving loxP sites on either side of exon 4. Cre-mediated recombination removed exon 4 and introduced a premature stop codon. Western blot analysis demonstrated that no detectable protein was present in homozygous E13.5 embryos. (J:211681)

Expression

In Mice Carrying this Mutation:

9 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rcor1 Mutation: 42 strains or lines available

References

• Original: J:211681 Yao H, et al., Corepressor Rcor1 is essential for murine erythropoiesis. Blood. 2014 May 15;123(20):3175-84
• All: 1 reference(s)