Ltf^{tm1(icre)Tdku}
Targeted Allele Detail

Summary

• Symbol: Ltf^{tm1(icre)Tdku}
• Name: lactotransferrin; targeted mutation 1, Takiko Daikoku
• MGI ID: MGI:5581966
• Synonyms: Lactoferrin-iCre, Ltf-cre, Ltf-iCre, Ltf^{tm1(icre,EGFP)Tdku}
• Gene: Ltf Location: Chr9:110848360-110871834 bp, + strand Genetic Position: Chr9, 60.79 cM, cytoband F
• Alliance: Ltf^{tm1(icre)Tdku} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211105
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Ltf^{tm1(icre)Tdku} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ltf (MGI:96837)

• Mutation details: A targeting construct was designed to replace part of exon1 and intron 1 of the gene with a codon optimized Cre recombinase gene (iCre) followed by an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), a polyadenylation site, and a frt-flanked neomycin/kanamycin resistance (neo/kan) cassette. (J:211105)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ltf (mouse) Summary of all recombinase alleles driven by Ltf. Comparative matrix view of recombinase activities Alliance mouse Ltf gene page

Expression

In Mice Carrying this Mutation:

12 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ltf Mutation: 41 strains or lines available

References

• Original: J:211105 Daikoku T, et al., Lactoferrin-iCre: A New Mouse Line to Study Uterine Epithelial Gene Function. Endocrinology. 2014 Jul;155(7):2718-24
• All: 27 reference(s)