Sec24d^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sec24d^{tm1c(EUCOMM)Hmgu}
• Name: SEC24 homolog D, COPII coat complex component; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5582296
• Synonyms: Sec24d-FL
• Gene: Sec24d Location: Chr3:123061104-123159290 bp, + strand Genetic Position: Chr3, 53.82 cM, cytoband G3
• Alliance: Sec24d^{tm1c(EUCOMM)Hmgu} page
• IMPC: Sec24d gene page

Mutation origin

• Mutant Cell Line: HEPD0753_7_H04
• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 123086679 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 123088075. The critical exon(s) is/are thus flanked by loxP sites. The lacZ and neomycin segments were excised with germline Actin-Flp recombinase, leaving exons 5 and 6 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sec24d Mutation: 59 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)