Tyr^em2Utr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tyr^em2Utr
• Name: tyrosinase; endonuclease-mediated mutation 2, University of Tsukuba Laboratory Animal Resource Center
• MGI ID: MGI:5582798
• Synonyms: Tyr^G291T
• Gene: Tyr Location: Chr7:87073979-87142637 bp, - strand Genetic Position: Chr7, 49.01 cM
• Alliance: Tyr^em2Utr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: Arginine codon 77 (CGT) in exon 1 was changed to leucine (CTT) (c.230G>T:p.R77L) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This allele was derived from mouse founder line #1 which also carries the 5-nucleotide-deletion allele Tyr^em1Utr in trans. (J:213052)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tyr Mutation: 379 strains or lines available

References

• Original: J:213052 Mizuno S, et al., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm Genome. 2014 Aug;25(7-8):327-34
• All: 2 reference(s)