Tg(Neurod1-cre/ERT2)M1Fcal
Transgene Detail

Summary

• Symbol: Tg(Neurod1-cre/ERT2)M1Fcal
• Name: transgene insertion M1, Federico Calegari
• MGI ID: MGI:5582823
• Synonyms: Neurod1-CreER^T2
• Transgene: Tg(Neurod1-cre/ERT2)M1Fcal Location: unknown
• Alliance: Tg(Neurod1-cre/ERT2)M1Fcal page

Transgene origin

• Strain of Origin: C57BL/6JOlaHsd

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Neurod1-cre/ERT2)M1Fcal expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Neurod1 (MGI:1339708)

• Mutation details: Bacterial artificial chromosome (BAC) library (Source BioScience) was used to obtain a 160 kb BAC (BMQ-305H2) containing the entire mouse Neurod1 gene. This BAC was modified to replace the coding region with a creERT2 fusion gene (a Cre recombinase fused to a human estrogen receptor ligand-binding domain) followed by a frt-flanked neomycin (neo) selection cassette. Four lines were generated: L (low expression), M1 (intermediate expression), M2, H (high expression). This is a representative record for all lines. Transgene expression patterns are consistent with endogenous gene expression. (J:213056)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Neurod1 (mouse) Summary of all recombinase alleles driven by Neurod1. Comparative matrix view of recombinase activities Alliance mouse Neurod1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:213056 Aprea J, et al., Generation and characterization of Neurod1-CreER(T2) mouse lines for the study of embryonic and adult neurogenesis. Genesis. 2014 Oct;52(10):870-8
• All: 4 reference(s)