Egln3^tm1Jomy
Targeted Allele Detail

Summary

• Symbol: Egln3^tm1Jomy
• Name: egl-9 family hypoxia-inducible factor 3; targeted mutation 1, Johanna Myllyharju
• MGI ID: MGI:5583030
• Synonyms: Hif-p4h-3^-
• Gene: Egln3 Location: Chr12:54225767-54250646 bp, - strand Genetic Position: Chr12, 22.9 cM
• Alliance: Egln3^tm1Jomy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213191
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Mutant mice were generated by replacing exon 2 with a neo-selection cassette flanked by loxP sites. RT-PCR verified that exon 2 is missing from the expressed transcript. The expressed protein is missing 2 amino acids required for binding of Fe2+ at the catalytic site, resulting in complete loss of the enzyme activity. (J:213191)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egln3 Mutation: 26 strains or lines available

References

• Original: J:213191 Laitala A, et al., Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis. Blood. 2012 Oct 18;120(16):3336-44
• All: 3 reference(s)