Dsel^tm1Mmac
Targeted Allele Detail

Summary

• Symbol: Dsel^tm1Mmac
• Name: dermatan sulfate epimerase-like; targeted mutation 1, Marco Maccarana
• MGI ID: MGI:5583872
• Gene: Dsel Location: Chr1:111786432-111792648 bp, - strand Genetic Position: Chr1, 50.84 cM
• Alliance: Dsel^tm1Mmac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213195
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced the coding exon containing the translation initation site. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts and skin. (J:213195)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dsel Mutation: 50 strains or lines available

References

• Original: J:213195 Bartolini B, et al., Mouse development is not obviously affected by the absence of dermatan sulfate epimerase 2 in spite of a modified brain dermatan sulfate composition. Glycobiology. 2012 Jul;22(7):1007-16
• All: 3 reference(s)