Tg(Vil1-mRFP1/TJP1)#Tjr
Transgene Detail

Summary

• Symbol: Tg(Vil1-mRFP1/TJP1)#Tjr
• Name: transgene insertion, Jerrold R Turner
• MGI ID: MGI:5584023
• Synonyms: villin-mRFP1-ZO-1
• Transgene: Tg(Vil1-mRFP1/TJP1)#Tjr Location: unknown
• Alliance: Tg(Vil1-mRFP1/TJP1)#Tjr page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: Mice bearing this transgene express a fusion protein composed of monomeric red fluorescent protein (mRFP) joined to the amino terminus of human tight junction protein 1 (TJP1; also called zonula occludens 1, ZO-1) in the intestinal villus epithelium under control of the regulatory elements of the mouse villin 1 (Vil1) gene. The transgene contains 9 kb of mouse Vil1, extending from 3.5 kb upstream of the transcription initiation site through the 5.5-kb first intron to the translation initiation codon in the second exon, followed by the coding sequence for mRFP1 joined to a human TJP1 cDNA and the polyadenylation signal of the bovine growth hormone gene. In-vivo imaging of the mucosal surface of externalized, opened jejunal segments of transgenic mice using a multiphoton confocal inverted microscope and a laser with a special emission range of 577-679 nm showed the mRFP1-TJP1 fusion protein concentrated at tight junctions of the intestinal epithelium; it is not associated with the lateral membrane. (J:53387, J:158798, J:213189)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:158798 Marchiando AM, et al., Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo. J Cell Biol. 2010 Apr 5;189(1):111-26
• All: 5 reference(s)