Gcm2^tm1.2Malx
Targeted Allele Detail

Summary

• Symbol: Gcm2^tm1.2Malx
• Name: glial cells missing homolog 2; targeted mutation 1.2, Michael Levine
• MGI ID: MGI:5584282
• Synonyms: Gcm2^deltaE2, Gcm2^tm1.1Mal2
• Gene: Gcm2 Location: Chr13:41254903-41264511 bp, - strand Genetic Position: Chr13, 20.31 cM
• Alliance: Gcm2^tm1.2Malx page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213334
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector contains a FRT-flanked neomycin selection cassette and a loxP site inserted downstream of exon 2 of the targeted gene, and another loxP site inserted upstream of exon 2. The neomycin cassette was deleted by transient transfection using flp recombinase. Cre-mediated recombination removed exon 2. The deletion creates a frameshift followed by a stop codon that results in the production of a short peptide lacking the main functional domains of the endogenous gene. (J:213334)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gcm2 Mutation: 34 strains or lines available

References

• Original: J:213334 Yuan Z, et al., Generation of mice encoding a conditional null allele of Gcm2. Transgenic Res. 2014 Aug;23(4):631-41
• All: 1 reference(s)