Gja1^tm3.1Clo
Targeted Allele Detail

Summary

• Symbol: Gja1^tm3.1Clo
• Name: gap junction protein, alpha 1; targeted mutation 3.1, Cecilia Lo
• MGI ID: MGI:5584311
• Synonyms: Cx43-MK4A, Gja1^tm1.1Beis
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^tm3.1Clo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212895
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A cassette containing the non-coding exon 1, and a neo/PGK cassette flanked by loxP sites followed by exon 2 that contains the whole Gja1 coding sequence including the mutations S255A, S262A, S279A, and S282A, was inserted into the endogenous locus. The neomycin selection cassette was removed by breeding with Tg(CMV-cre)1Cgn mice. These serine to alanine mutations prevent Gja1 phosphorylation by mitogen-activated protein kinase. (J:212895)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 60 strains or lines available

References

• Original: J:212895 Johnstone SR, et al., MAPK phosphorylation of connexin 43 promotes binding of cyclin E and smooth muscle cell proliferation. Circ Res. 2012 Jul 6;111(2):201-11
• All: 3 reference(s)