Isca1tm1b(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Isca1tm1b(KOMP)Wtsi |
Name: |
iron-sulfur cluster assembly 1; targeted mutation 1b, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:5585595 |
Synonyms: |
Isca1- |
Gene: |
Isca1 Location: Chr13:59903229-59917603 bp, - strand Genetic Position: Chr13, 31.88 cM, cytoband A3.1
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Alliance: |
Isca1tm1b(KOMP)Wtsi page
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IMPC: |
Isca1 gene page |
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Isca1tm1b(KOMP)Wtsi/Isca1tm1b(KOMP)Wtsi mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos are smaller and form a rudimentary egg cylinder but no primitive streak or hallmarks of gastrulation are seen at E7.5.
Show the 1 phenotype image(s) involving this allele.
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Mutant Cell Line: |
EPD0855_4_A09 |
Germline Transmission: |
Earliest citation of germline transmission:
J:188991
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 59906420 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 59907262. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision deleted the critical sequence and neomycin selection cassette yielding a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:148605, J:173534, J:188991)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012; |
All: |
7 reference(s) |
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