Tyr^tm1Arte
Targeted Allele Detail

Summary

• Symbol: Tyr^tm1Arte
• Name: tyrosinase; targeted mutation 1, TaconicArtemis
• MGI ID: MGI:5585631
• Gene: Tyr Location: Chr7:87073979-87142637 bp, - strand Genetic Position: Chr7, 49.01 cM
• Alliance: Tyr^tm1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213411
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: Guanosine was replaced by cytosine at nucleic acid position 308 of the tyrosinase gene, in the first exon, replicating the inactivating Tyr^c mutation that results in substitution of serine for cysteine at amino acid position 103 of the tyrosinase protein; a puromycin resistance cassette flanked by frt sequences was inserted into intron 1. The selection cassette was excised by flp recombinase, leaving a single frt site in the intron. (J:213411)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tyr Mutation: 379 strains or lines available

Notes

The frt-flanked selection cassette was deleted in vitro in the targeted ES cells by transient transfection with a flpe expression vector. (J:213411)

References

• Original: J:213411 Zevnik B, et al., C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 2014;9(3):e90570
• All: 2 reference(s)