Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag}
• Name: collagen, type I, alpha 1; targeted mutation 3, Erwin F Wagner
• MGI ID: MGI:5585716
• Synonyms: tet-O-Fosl1, tet-O-Fra1
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213764
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fosl1 (MGI:107179)

• Mutation details: KH2 ES cells were retargeted with a "flp-in plasmid containing a tetracycline responsive element (TRE or tetO), FLAG-tagged Fosl1 cDNA sequence, GtX IRES sequence and DsRed reporter. A flpe-recombinase plasmid facilitated integration into the 3' UTR region. (J:213764)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:213764 Hasenfuss SC, et al., Activator Protein 1 transcription factor Fos-related antigen 1 (Fra-1) is dispensable for murine liver fibrosis, but modulates xenobiotic metabolism. Hepatology. 2014 Jan;59(1):261-73
• All: 3 reference(s)