Ttc21b^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ttc21b^{tm2c(KOMP)Wtsi}
• Name: tetratricopeptide repeat domain 21B; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5587034
• Synonyms: Thm1^flox
• Gene: Ttc21b Location: Chr2:66014671-66086961 bp, - strand Genetic Position: Chr2, 39.08 cM
• Alliance: Ttc21b^{tm2c(KOMP)Wtsi} page
• IMPC: Ttc21b gene page

Mutation origin

• Mutant Cell Line: EPD0041_2_E09
• Germline Transmission: Earliest citation of germline transmission: J:213263
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 66079515 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 66080377. The critical exon, exon 4, is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele, removing the lacZ/neomycin cassette, but leaving exon 4 flanked by loxP sites. (J:213263)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttc21b Mutation: 52 strains or lines available

References

• Original: J:213263 Tran PV, et al., Downregulating hedgehog signaling reduces renal cystogenic potential of mouse models. J Am Soc Nephrol. 2014 Oct;25(10):2201-12
• All: 7 reference(s)