Snhg14^tm1Alb
Targeted Allele Detail

Summary

• Symbol: Snhg14^tm1Alb
• Name: small nucleolar RNA host gene 14; targeted mutation 1, Arthur L Beaudet
• MGI ID: MGI:5587826
• Synonyms: Ube3a^ATS-stop
• Gene: Snhg14 Location: Chr7:58957672-59625507 bp, - strand Genetic Position: Chr7, 34.04 cM
• Alliance: Snhg14^tm1Alb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207499
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted (Not Applicable) Mutation: Insertion Mutation details: A triple polyadenylation sequence and neomycin resistance cassette were inserted into the 3' end upstream of the Ube3a antisense transcript (chr7:66573289 NCBI37/mm9). The selection cassette is in the opposite orientation. qPCR confirmed the absence of transcript beyond the insertion site expression and unsilenced Ube3a transcript expression when the allele is inherited paternally. Maternal transcript expression is not affected. (J:207499)

Generation of the Snhg14^tm1Alb allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Snhg14 Mutation: 3 strains or lines available

References

• Original: J:207499 Meng L, et al., Truncation of Ube3a-ATS unsilences paternal Ube3a and ameliorates behavioral defects in the Angelman syndrome mouse model. PLoS Genet. 2013 Dec;9(12):e1004039
• All: 1 reference(s)