S1pr1^{tm3.1(tTA,-Arrb2)Rlp}
Targeted Allele Detail

Summary

• Symbol: S1pr1^{tm3.1(tTA,-Arrb2)Rlp}
• Name: sphingosine-1-phosphate receptor 1; targeted mutation 1, Richard L Proia
• MGI ID: MGI:5588124
• Synonyms: S1P1-beta-arrestin
• Gene: S1pr1 Location: Chr3:115504082-115508704 bp, - strand Genetic Position: Chr3, 50.0 cM
• Alliance: S1pr1^{tm3.1(tTA,-Arrb2)Rlp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212772
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Transactivator)
• Mutation: Insertion
• Mutation details: A targeting vector was designed with two fusion proteins separated by an internal ribosome entry site (IRES). One fusion protein contains a tobacco etch virus (TEV) protease recognition sequence (tevs) fused to a tetracycline-regulated transactivator (tTA or "Tet-Off") sequence. The second contains a murine arrestin, beta 2 (Arrb2) gene fused to the TEV protease. This construct was inserted at the 3 end of exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene and was followed by a polyadenylation sequence and a loxP-flanked neomycin (neo) resistance cassette. Cre-mediated recombination removed the selection cassette. (J:212772)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any S1pr1 Mutation: 32 strains or lines available

References

• Original: J:212772 Kono M, et al., Sphingosine-1-phosphate receptor 1 reporter mice reveal receptor activation sites in vivo. J Clin Invest. 2014 May 1;124(5):2076-86
• All: 4 reference(s)