Nrp1^tm1.1Izac
Targeted Allele Detail

Summary

• Symbol: Nrp1^tm1.1Izac
• Name: neuropilin 1; targeted mutation 1.1, Ian Zachary
• MGI ID: MGI:5588167
• Synonyms: Nrp1^Y297A
• Gene: Nrp1 Location: Chr8:129085085-129229844 bp, + strand Genetic Position: Chr8, 75.78 cM, cytoband E
• Alliance: Nrp1^tm1.1Izac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208348
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with a modified one in which nucleotide substitutions (TAT to GCT) result in the amino acid substitution of alanine for tyrosine at position 297 (Y297A). This mutation reduces VEGF164 binding. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of the modified exon 2. Immunoblotting confirmed severely reduced protein expression in the brain, heart and kidney. (J:208348)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nrp1 Mutation: 84 strains or lines available

References

• Original: J:208348 Fantin A, et al., Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis. Development. 2014 Feb;141(3):556-62
• All: 3 reference(s)