Prss8^tm1.1Bug
Targeted Allele Detail

Summary

• Symbol: Prss8^tm1.1Bug
• Name: serine protease 8 (prostasin); targeted mutation 1.1, Thomas H Bugge
• MGI ID: MGI:5588579
• Synonyms: Prss8^Cat-
• Gene: Prss8 Location: Chr7:127524889-127529266 bp, - strand Genetic Position: Chr7, 69.84 cM
• Alliance: Prss8^tm1.1Bug page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214122
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A T to G nucleotide substitution was introduced into exon 6 at nucleotide 943 by homologous recombination. This point mutation causes the substitution of the active site serine 238 within the catalytic histidine-aspartate-serine triad with alanine (S238A) and renders it catalytically inactive. In addition, a loxP flanked neomycin cassette under the control of the 3-phosphoglycerate kinase promoter was inserted upstream of the substitution. The neomycin cassette was removed via Cre-mediated recombination by mating with Tg(EIIa-cre)C5379Lmgd transgenic mice. RT-PCR indicates that the introduction of the point mutation and the loxP site in intron 5 do not affect mRNA expression. Western blot analysis shows that protein is expressed at levels similar to wild-type and the presence of a faster migrating species in the kidney and lung. (J:214122)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prss8 Mutation: 24 strains or lines available

References

• Original: J:214122 Peters DE, et al., The membrane-anchored serine protease prostasin (CAP1/PRSS8) supports epidermal development and postnatal homeostasis independent of its enzymatic activity. J Biol Chem. 2014 May 23;289(21):14740-9
• All: 3 reference(s)