Fezf1^{tm1.1(cre/folA)Hze}
Targeted Allele Detail

Summary

• Symbol: Fezf1^{tm1.1(cre/folA)Hze}
• Name: Fez family zinc finger 1; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5603434
• Synonyms: Fezf1-2A-dCre, Fezf1-2A-DHFR-Cre-D, Fezf1-2A-ecDHFR^R12Y/Y100I/Cre-D
• Gene: Fezf1 Location: Chr6:23245043-23248361 bp, - strand Genetic Position: Chr6, 10.55 cM, cytoband A3
• Alliance: Fezf1^{tm1.1(cre/folA)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Fezf1^{tm1.1(cre/folA)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fezf1 (MGI:1920441)

• Mutation details: ES cells previously targeted with a T2A-Cre vector immediately downstream of the Fez family zinc finger 1 translational STOP codon were re-targeted with a "T2A-hDHFR/Cre" vector and a FLP-expressing plasmid to facilitate recombination. Correctly re-targeted ES cells had (from 5' to 3') a partial Fezf1 intron 3 sequence containing an frt3 site, a partial Fezf1 exon 4 sequence up to (but not including) the endogenous stop codon, a T2A cleavage sequence that is in-frame with the Fezf1 coding sequence, a dCre fusion gene (described below) that is in-frame with the Fezf1 coding sequence, a bovine growth hormone polyA sequence, an AttB sequence, a PGK-hygromycin-SV40polyA cassette (with an mRNA spice donor-frt5 site-mRNA spice acceptor in the hygromycin gene), and an AttP sequence. The dCre fusion gene (also called destabilized Cre, DHFR/Cre or ecDHFR/Cre) is Cre recombinase fused at its N terminus to the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. PhiC31-mediation recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL). (J:146821)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fezf1 (mouse) Summary of all recombinase alleles driven by Fezf1. Alliance mouse Fezf1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fezf1 Mutation: 31 strains or lines available

References

• Original: J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06
• All: 6 reference(s)