Hand1^tm3Abfi
Targeted Allele Detail

Summary

• Symbol: Hand1^tm3Abfi
• Name: heart and neural crest derivatives expressed 1; targeted mutation 3, Anthony B Firulli
• MGI ID: MGI:5604130
• Synonyms: Hand1^{stopfloxHand1T107;S109A}, PO4-
• Gene: Hand1 Location: Chr11:57719539-57722973 bp, - strand Genetic Position: Chr11, 35.27 cM
• Alliance: Hand1^tm3Abfi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214092
• Parent Cell Line: CCE916 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Residues T107 and S109 were replaced with alanines. A Stop-flox cassette containing a neomycin resistance gene was also inserted downstream of the Hand1 transcriptional start site, but upstream of the Hand1-initiating methionine. Cre-excision of the stop cassette results in expression of the mutant. (J:214092)

Expression

In Structures Affected by this Mutation:

21 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hand1 Mutation: 15 strains or lines available

References

• Original: J:214092 Firulli BA, et al., Hand1 phosphoregulation within the distal arch neural crest is essential for craniofacial morphogenesis. Development. 2014 Aug;141(15):3050-61
• All: 2 reference(s)