Gt(ROSA)26Sor^{tm1(Neurog3*S183A*S187A)Axbe}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Neurog3*S183A*S187A)Axbe}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Axel Behrens
• MGI ID: MGI:5604877
• Synonyms: R26-LSL-Ngn3-AA, Rosa26-Ngn3-AA-IRES-eGFP-pA+, Rosa26-Ngn3-AA-IRESeGFP-pA+
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Neurog3*S183A*S187A)Axbe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215154
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: Mice with this conditional insertion, upon cre recombinase expression, produce a mutant neurogenin 3 protein in which serine has been replaced by alanine at amino acid positions 183 and 187 (S183A/S187A), eliminating a glycogen synthase kinase 3 beta (GSK3beta) consensus phosphorylation site, impairing its association with FBW7 and stabilizing the protein. The targeting construct was made by insertion of a cDNA encoding the full-length mouse protein with the introduced mutations into the Gateway compatible pROSA26-DV1 plasmid. The final construct contains a splice acceptor followed by a loxP-flanked Pgk-neo^r with a triple polyadenylation signal (STOP sequence), the modified Neurog3 cDNA, and an enhanced green fluorescent protein (EGFP) cDNA downstream of an internal ribosomal entry site (IRES). Deletion of the Pgk-neo^r-STOP by cre recombinase results in co-expression of NEUROG3-S183A/S187A and EGFP. (J:194078, J:215154)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:215154 Sancho R, et al., Loss of Fbw7 reprograms adult pancreatic ductal cells into alpha, delta, and beta cells. Cell Stem Cell. 2014 Aug 7;15(2):139-53
• All: 2 reference(s)