Ift27^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ift27^{tm1c(EUCOMM)Hmgu}
• Name: intraflagellar transport 27; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5605398
• Synonyms: Ift27^flox
• Gene: Ift27 Location: Chr15:78043660-78058281 bp, - strand Genetic Position: Chr15, 36.92 cM, cytoband E2
• Alliance: Ift27^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ift27 gene page

Mutation origin

• Mutant Cell Line: HEPD0653_7_E10
• Germline Transmission: Earliest citation of germline transmission: J:215449
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 78049209 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 78051786. The critical exon(s) is/are thus flanked by loxP sites. The "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele. (J:157065)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ift27 Mutation: 18 strains or lines available

Notes

Cell line HEPD0653_7_E10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:215449

References

• Original: J:215449 Eguether T, et al., IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment. Dev Cell. 2014;31:279-290
• All: 4 reference(s)