Gt(ROSA)26Sor^tm2.2Ksvo
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm2.2Ksvo
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.2, Keral Svoboda
• MGI ID: MGI:5605725
• Synonyms: R26 LSL-CAG-LSL-ReaChR-mCit, Rosa26 CAG-LSL-ReaChR-mCit
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm2.2Ksvo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217788
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A CAG promoter (cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter), FRT-flanked stop cassette, loxP-flanked stop cassette, ReaChR red-shifted channelrhodopsin, fluorescent reporter mCitrine, woodchuck hepatitis post-transcriptional regulatory element (WPRE), poly A (pA) tail, and AttB/AttP-flanked neomycin resistance cassette were introduced to intron 1 of the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neomycin cassette. Flp-mediated recombination removed the FRT-flanked stop cassette. (J:217788)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:217788 Hooks BM, et al., Dual-channel circuit mapping reveals sensorimotor convergence in the primary motor cortex. J Neurosci. 2015 Mar 11;35(10):4418-26
• All: 14 reference(s)