Rnf216^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnf216^{tm1b(EUCOMM)Wtsi}
• Name: ring finger protein 216; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5605760
• Synonyms: Rnf216^-
• Gene: Rnf216 Location: Chr5:142976648-143098749 bp, - strand Genetic Position: Chr5, 81.86 cM, cytoband G2
• Alliance: Rnf216^{tm1b(EUCOMM)Wtsi} page
• IMPC: Rnf216 gene page

Mutation origin

• Mutant Cell Line: EPD0789_2_E09
• Germline Transmission: Earliest citation of germline transmission: J:204739
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Cre-mediated excision of the parental Rnf216^{tm1a(EUCOMM)Wtsi} allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exons 4 and 5 leaving behind the inserted lacZ reporter sequence. Western blot analysis confirmed the absence of protein expression in the brain and testes of homozygous mutant mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:204739, J:274674)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rnf216 Mutation: 53 strains or lines available

References

• Original: J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023
• All: 5 reference(s)