Cnot3^tm1.1Tya
Targeted Allele Detail

Summary

• Symbol: Cnot3^tm1.1Tya
• Name: CCR4-NOT transcription complex, subunit 3; targeted mutation 1.1, Tadashi Yamamoto
• MGI ID: MGI:5607530
• Synonyms: Cnot3^-
• Gene: Cnot3 Location: Chr7:3648267-3664108 bp, + strand Genetic Position: Chr7, 2.1 cM
• Alliance: Cnot3^tm1.1Tya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:180033
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo cassette flanked by two loxP sites was inserted in the intron preceding exon 2 and another loxP site was inserted following exon 9. Both the PGK-neo cassette and exons 2-9 were removed via cre-mediated recombination. (J:180033)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Cnot3^tm1.1Tya/Cnot3^tm1.1Tya	B6.129P2(Cg)-Cnot3^tm1.1Tya
ht2	Cnot3^tm1.1Tya/Cnot3^+	B6.129P2(Cg)-Cnot3^tm1.1Tya
cx3	Cnot3^tm1.1Tya/Cnot3^+ Lep^ob/Lep^ob	involves: 129P2/OlaHsd * C57BL/6J

Phenotypes:

Affected Systems	hm1	ht2	cx3
show or hide all annotated terms Sex:
adipose tissue		√
decreased subcutaneous adipose tissue amount		√
decreased body fat mass		√
decreased brown fat cell size		√
decreased white fat cell size		√
decreased percent body fat/body weight		√
behavior/neurological			N
behavior/neurological phenotype			N
cellular		√
abnormal osteoclast differentiation		√
growth/size/body		√	√
decreased body fat mass		√
decreased birth body size		√
decreased body weight		√	√
decreased body length		√
decreased susceptibility to diet-induced obesity		√
hematopoietic system		√
abnormal osteoclast differentiation		√
increased osteoclast cell number		√
homeostasis/metabolism		√	√
decreased susceptibility to diet-induced obesity		√
decreased circulating glucose level		√
decreased fasting circulating glucose level		√
decreased circulating triglyceride level		√
increased oxygen consumption		√	√
decreased respiratory quotient			√
improved glucose tolerance		√	√
increased insulin sensitivity		√	√
immune system		√
abnormal osteoclast differentiation		√
increased osteoclast cell number		√
integument		√
decreased subcutaneous adipose tissue amount		√
liver/biliary system		√
small liver		√
decreased susceptibility to diet-induced hepatic steatosis		√
mortality/aging	√
embryonic lethality, complete penetrance	√
skeleton		√
increased osteoclast cell number		√
decreased bone mineral density		√
decreased compact bone thickness		√
abnormal trabecular bone morphology		√
decreased bone trabecula number		√
decreased trabecular bone mass		√
decreased trabecular bone thickness		√
osteoporosis		√
abnormal skeleton development		√
abnormal osteoclast differentiation		√
abnormal bone mineralization		√
increased bone resorption		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnot3 Mutation: 35 strains or lines available

References

• Original: J:180033 Morita M, et al., Obesity resistance and increased hepatic expression of catabolism-related mRNAs in Cnot3+/- mice. EMBO J. 2011 Nov 16;30(22):4678-91
• All: 3 reference(s)