Mpl^tm2.1Wsa
Targeted Allele Detail

Summary

• Symbol: Mpl^tm2.1Wsa
• Name: myeloproliferative leukemia virus oncogene; targeted mutation 2.1, Warren S Alexander
• MGI ID: MGI:5607579
• Synonyms: Mpl^fl
• Gene: Mpl Location: Chr4:118299612-118314710 bp, - strand Genetic Position: Chr4, 54.61 cM, cytoband D
• Alliance: Mpl^tm2.1Wsa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208120
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: An IRES-GFP-FRT-PGK-neo-FRT-loxP cassette was inserted immediately downstream of the stop codon in exon 12, along with a loxP site in intron 10. The PGK-neo cassette was removed via Flp-mediated recombination, leaving exons 11-12 and the IRES-GFP floxed. Expression of GFP was confirmed by flow cytometry and live cell imaging in megakaryocyte progenitor cells, megakaryoctyes, and LSK cells. (J:208120)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpl Mutation: 48 strains or lines available

References

• Original: J:208120 Ng AP, et al., Mpl expression on megakaryocytes and platelets is dispensable for thrombopoiesis but essential to prevent myeloproliferation. Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5884-9
• All: 1 reference(s)