Penk^tm2(cre)Hze
Targeted Allele Detail

Summary

• Symbol: Penk^tm2(cre)Hze
• Name: preproenkephalin; targeted mutation 2, Hongkui Zeng
• MGI ID: MGI:5607585
• Synonyms: Penk-IRES2-Cre
• Gene: Penk Location: Chr4:4133536-4138815 bp, - strand Genetic Position: Chr4, 2.31 cM
• Alliance: Penk^tm2(cre)Hze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Penk^tm2(cre)Hze expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Penk (MGI:104629)

• Mutation details: The targeting vector contains from 5' to 3', a partial Penk sequence spanning exon 1 to intron 1 (with an frt3 site in intron 1), a partial Penk exon 2 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene (Hygro2) with SV40 late polyA signal, and an AttP site. (J:146821)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Penk (mouse) Summary of all recombinase alleles driven by Penk. Alliance mouse Penk gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Penk Mutation: 22 strains or lines available

References

• Original: J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06
• All: 27 reference(s)