Plcl1^tm2.1Matk
Targeted Allele Detail

Summary

• Symbol: Plcl1^tm2.1Matk
• Name: phospholipase C-like 1; targeted mutation 2.1, Matilda Katan
• MGI ID: MGI:5607952
• Synonyms: Plce^RAm
• Gene: Plcl1 Location: Chr1:55445080-55793444 bp, + strand Genetic Position: Chr1, 28.15 cM
• Alliance: Plcl1^tm2.1Matk page

Increase in TPA-induced skin proliferation in Plcl1^tm1.1Matk/Plcl1^tm1.1Matk and Plcl1^tm2.1Matk/Plcl1^tm2.1Matk mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207384
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Three point mutations (R2130L, K2151E, and Y2154L) were introduced into exons 29 and 30 and an FRT flanked selection cassette was inserted via homologous recombination. Flp mediated recombination removed the neo cassette. The point mutations disrupt the Ras binding domain in the RA2 domain of the protein. Quantitative RT-PCR indicates that mRNA expression in homozygous mice is similar to wild-type controls. (J:207384)

Generation of the Plcl1^tm2.1Matk allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plcl1 Mutation: 54 strains or lines available

References

• Original: J:207384 Martins M, et al., Tumor suppressor role of phospholipase C{varepsilon} in Ras-triggered cancers. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4239-44
• All: 1 reference(s)