Ptbp2^tm1.2Dblk
Targeted Allele Detail

Summary

• Symbol: Ptbp2^tm1.2Dblk
• Name: polypyrimidine tract binding protein 2; targeted mutation 2.1, Douglas Black
• MGI ID: MGI:5608588
• Gene: Ptbp2 Location: Chr3:119512391-119578115 bp, - strand Genetic Position: Chr3, 52.18 cM
• Alliance: Ptbp2^tm1.2Dblk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207976
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An FRT-flanked PGK-neomycin cassette and loxP site were introduced to intron 3 and a loxP site was placed in intron 4 via homologous recombination. The neomycin cassette was excised from resultant mice through crosses with a Flp recombinase-expressing strain, leaving exon 4 flanked by loxP sites. Cre-mediated recombination removed exon 4. Immunoblot confirms the absence of protein. (J:207976)

Expression

In Mice Carrying this Mutation:	14 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptbp2 Mutation: 54 strains or lines available

References

• Original: J:207976 Li Q, et al., The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation. Elife. 2014;3:e01201
• All: 5 reference(s)