Rhoj^tm1.2Auem
Targeted Allele Detail

Summary

• Symbol: Rhoj^tm1.2Auem
• Name: ras homolog family member J; targeted mutation 1.2, Akiyoshi Uemura
• MGI ID: MGI:5608669
• Synonyms: Rhoj^GFP
• Gene: Rhoj Location: Chr12:75355096-75448230 bp, + strand Genetic Position: Chr12, 32.63 cM
• Alliance: Rhoj^tm1.2Auem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208154
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing a floxed mouse Rhoj cDNA, poly A, EGFP, poly A and an FRT flanked SV40-Neo replaced exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette. Cre mediated recombination removed the Rhoj cDNA allowing expression of the EGFP reporter. (J:208154)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhoj Mutation: 16 strains or lines available

References

• Original: J:208154 Kim C, et al., Vascular RhoJ is an effective and selective target for tumor angiogenesis and vascular disruption. Cancer Cell. 2014 Jan 13;25(1):102-17
• All: 4 reference(s)