Abcc2^{tm1.1(ABCC2)Arte}
Targeted Allele Detail

Summary

• Symbol: Abcc2^{tm1.1(ABCC2)Arte}
• Name: ATP-binding cassette, sub-family member 2; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:5609256
• Synonyms: hMRP2, huMRP2
• Gene: Abcc2 Location: Chr19:43770747-43826771 bp, + strand Genetic Position: Chr19, 36.67 cM
• Alliance: Abcc2^{tm1.1(ABCC2)Arte} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216646
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Abcc2^{tm1.1(ABCC2)Arte} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ABCC2 (1244)

  Abcc2

  (MGI:1352447)

• Mutation details: Exon 2 was replaced with a cDNA containing human ABCC2 exons 2-32 and a polyA signal and a neo cassette was inserted via homologous recombination. Flp mediated recombination removed the neo cassette. Quantitative PCR analysis confirmed absence of expression of the mouse gene and stable expression of the human gene in the liver kidney and intestines of homozygous mice. Expression levels of the human gene are elevated compared to expression levels of the mouse gene. Western blot analysis confirmed the expression of the human protein and absence of the mouse protein in the liver, kidney and intestines from homozygous mice. (J:216646)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abcc2 Mutation: 90 strains or lines available

References

• Original: J:216646 Scheer N, et al., Generation and characterization of a novel multidrug resistance protein 2 humanized mouse line. Drug Metab Dispos. 2012 Nov;40(11):2212-8
• All: 2 reference(s)